SOCS4 Rabbit Polyclonal Antibody
cat.: HA500307
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SOCS4 aa 391-440. |
| Positive control: | Rat brain tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, Hela, human colon carcinoma tissue. |
| Subcellular location: | Phosphatidylinositol 3-kinase complex. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:200 1200 |
| Uniprot #: | SwissProt: Q8WXH5 Human | Q91ZA6 Mouse | B5DF08 Rat |
| Alternative names: | CIS4 Cish4 Cytokine inducible SH2 protein 4 DKFZp686J1568 HSPC060 SH2 domain containing SOCS box protein SOCS-4 SOCS-7 SOCS4 SOCS4_HUMAN SOCS6 SOCS7 SSI4 STAI4 STAT induced STAT inhibitor 4 STAT4 STATI4 Suppressor of cytokine signaling 4 Suppressor of cytokine signaling 7 |
Images
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Fig1:
Western blot analysis of SOCS4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500307, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Predicted band size: 51 kDa Observed band size: 51 kDa |
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Fig2: ICC staining of SOCS4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500307, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-SOCS4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500307, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.