AMHR2 Rabbit Polyclonal Antibody
cat.: HA500309
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 63/52 kDa
Isotype: IgG
Immunogen: Recombinant protein within human AMHR2 aa 350-573 / 573.
Positive control: MCF-7 cell lysate, Hela cell lysate, 293 cell lysate, mouse lung tissue lysate, MCF-7, rat uterus tissue, human lung carcinoma tissue, human uterus tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:100
1:300-1:800
Uniprot #: SwissProt: Q16671 Human | Q8K592 Mouse | Q62893 Rat
Alternative names: AMH type II receptor AMHR AMHR2 AMHR2_HUMAN Anti Mullerian hormone receptor, type II Anti-Muellerian hormone type II receptor Anti-Muellerian hormone type-2 receptor MGC141312 MIS type II receptor MISR2 MISRII MRII Muellerian hormone type 2 receptor Muellerian hormone type II receptor Muellerian inhibiting substance type II receptor Mullerian hormone receptor type II Mullerian inhibiting substance type II receptor
Images
HA500309_1.jpg Fig1: Western blot analysis of AMHR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500309, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate
Lane 3: 293 cell lysate
Lane 4: Mouse lung tissue lysate
HA500309_2.jpg Fig2: ICC staining of AMHR2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500309, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500309_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-AMHR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500309, 1/300) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500309_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-AMHR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500309, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500309_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-AMHR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500309, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.