p114RhoGEF Rabbit Polyclonal Antibody
cat.: HA500310
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 152 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human p114RhoGEF aa 1000-1200 / 1361. |
| Positive control: | HepG2 cell lysate, Hela cell lysate, 293 cell lysate, PC-3 cell lysate, Daudi cell lysate, rat kidney tissue, Hela. |
| Subcellular location: | Cytoskeleton, Cell membrane, Apical cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:400 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q6ZSZ5 Human | F1LT94 Rat |
| Alternative names: | 114 kDa Rho specific guanine nucleotide exchange factor 114 kDa Rho-specific guanine nucleotide exchange factor ARHGEF 18 Arhgef18 ARHGI_HUMAN KIAA0521 MGC15913 p114 Rho GEF p114-Rho-GEF p114RhoGEF Rho guanine nucleotide exchange factor 18 Rho specific guanine nucleotide exchange factor p114 Rho/Rac guanine nucleotide exchange factor (GEF) 18 Rho/Rac guanine nucleotide exchange factor 18 SA RhoGEF SA-RhoGEF Septin associated RhoGEF Septin-associated RhoGEF |
Images
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Fig1:
Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500310, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293 cell lysate Lane 4: PC-3 cell lysate Lane 5: Daudi cell lysate Predicted band size: 152 kDa Observed band size: 114/152 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-p114RhoGEF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500310, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of p114RhoGEF was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500310, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.