p114RhoGEF Rabbit Polyclonal Antibody
cat.: HA500310
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 152 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human p114RhoGEF aa 1000-1200 / 1361. |
| Positive control: | HepG2 cell lysate, Hela cell lysate, 293 cell lysate, PC-3 cell lysate, Daudi cell lysate. |
| Subcellular location: | Cytoskeleton, Cell membrane, Apical cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q6ZSZ5 Human | F1LT94 Rat |
| Alternative names: | 114 kDa Rho specific guanine nucleotide exchange factor 114 kDa Rho-specific guanine nucleotide exchange factor ARHGEF 18 Arhgef18 ARHGI_HUMAN KIAA0521 MGC15913 p114 Rho GEF p114-Rho-GEF p114RhoGEF Rho guanine nucleotide exchange factor 18 Rho specific guanine nucleotide exchange factor p114 Rho/Rac guanine nucleotide exchange factor (GEF) 18 Rho/Rac guanine nucleotide exchange factor 18 SA RhoGEF SA-RhoGEF Septin associated RhoGEF Septin-associated RhoGEF |
Images
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Fig1:
Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500310, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293 cell lysate Lane 4: PC-3 cell lysate Lane 5: Daudi cell lysate Predicted band size: 152 kDa Observed band size: 114/152 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.