Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 48 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human BMP10 aa 100-310 / 424. |
Positive control: | MCF-7 cell lysate, 293 cell lysate, rat liver tissue lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: O95393 Human | Q4AEG6 Rat |
Alternative names: | BMP-10 Bmp10 BMP10_HUMAN Bone morphogenetic protein 10 Bone morphogenetic protein 10 preproprotein MGC126783 OTTHUMP00000159973 |
Fig1:
Western blot analysis of BMP10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500312, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 3: Rat liver tissue lysate |