Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 48 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human BMP10 aa 100-310 / 424. |
Positive control: | NCI-H1299 cell lysate, MCF7 cell lysate, 293T cell lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: O95393 Human |
Alternative names: | BMP-10 Bmp10 BMP10_HUMAN Bone morphogenetic protein 10 Bone morphogenetic protein 10 preproprotein MGC126783 OTTHUMP00000159973 |
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Fig1:
Western blot analysis of BMP10 on different lysates with Rabbit anti-BMP10 antibody (HA500312) at 1/2,000 dilution. Lane 1: NCI-H1299 cell lysate Lane 2: MCF7 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500312) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |