BMP10 Rabbit Polyclonal Antibody
cat.: HA500312
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within human BMP10 aa 100-310 / 424.
Positive control: NCI-H1299 cell lysate, MCF7 cell lysate, 293T cell lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: O95393 Human
Alternative names: BMP-10 Bmp10 BMP10_HUMAN Bone morphogenetic protein 10 Bone morphogenetic protein 10 preproprotein MGC126783 OTTHUMP00000159973
Images
HA500312_1.jpg Fig1: Western blot analysis of BMP10 on different lysates with Rabbit anti-BMP10 antibody (HA500312) at 1/2,000 dilution.

Lane 1: NCI-H1299 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: 293T cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 55 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500312) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.