| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BMP10 aa 100-310 / 424. |
| Positive control: | NCI-H1299 cell lysate, MCF7 cell lysate, 293T cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: O95393 Human |
| Alternative names: | BMP-10 Bmp10 BMP10_HUMAN Bone morphogenetic protein 10 Bone morphogenetic protein 10 preproprotein MGC126783 OTTHUMP00000159973 |
|
Fig1:
Western blot analysis of BMP10 on different lysates with Rabbit anti-BMP10 antibody (HA500312) at 1/2,000 dilution. Lane 1: NCI-H1299 cell lysate Lane 2: MCF7 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500312) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |