CCNT1 Rabbit Polyclonal Antibody
cat.: HA500313
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 81 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CCNT1 aa 275-535 / 726. |
| Positive control: | K562 cell lysate, Hela cell lysate, HL-60 cell lysates. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: O60563 Human |
| Alternative names: | CCN T1 CCNT CCNT 1 Ccnt1 CCNT1_HUMAN CDK9 associated C type protein Cyc T1 Cyclin C related protein Cyclin T cyclin T1 Cyclin T1b Cyclin-T Cyclin-T1 CYCT 1 CycT1 HIVE1 Human immunodeficiency virus 1 expression Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 pTEFb subunit Subunit of positive elongation transcription factor b |
Images
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Fig1:
Western blot analysis of CCNT1 on different lysates with Rabbit anti-CCNT1 antibody (HA500313) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CCNT1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500313) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CCNT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500313, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Hela cell lysate |
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Fig3: Western blot analysis of CCNT1 on HL-60 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500313, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.