AP2B1 Rabbit Polyclonal Antibody
cat.: HA500315
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 104/106/98 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AP2B1 aa 400-600 / 937. |
| Positive control: | A549 cell lysate, PC-3M cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat kidney tissue, human liver tissue, A549. |
| Subcellular location: | Cell membrane, Coated pit, Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:5,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P63010 Human | Q9DBG3 Mouse | P62944 Rat |
| Alternative names: | Adapter related protein complex 2 beta 1 subunit Adapter-related protein complex 2 beta subunit Adaptin, beta 2 (beta) Adaptor protein complex AP-2 subunit beta Adaptor related protein complex 2, beta 1 subunit ADTB2 AP-2 complex subunit beta AP105B AP2 BETA Ap2b1 AP2B1_HUMAN Beta adaptin Beta-2-adaptin Beta-adaptin Beta2 adaptin CLAPB1 Clathrin assembly protein complex 2 beta large chain Clathrin associated/assembly/adaptor protein, large, beta 1 DKFZp781K0743 Plasma membrane adaptor HA2/AP2 adaptin beta subunit |
Images
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Fig1:
Western blot analysis of AP2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500315, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: PC-3M cell lysate |
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Fig2:
Western blot analysis of AP2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500315, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-AP2B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500315, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AP2B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500315, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of AP2B1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500315, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.