GRK3 Rabbit Polyclonal Antibody
cat.: HA500317
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 80 kDa
Isotype: IgG
Immunogen: Recombinant protein within human GRK3 aa 450-688 / 688.
Positive control: Jurkat cell lysate, SHSY5Y cell lysate, HL-60 cell lysate, rat stomachl tissue lysate, MG-63, SiHa, SH-SY5Y.
Subcellular location: cytosol, plasma membrane.
Recommended Dilutions:
  WB
  IF-Cell
  FC

1:1,000
1:50-1:200
1:500-1,000
Uniprot #: SwissProt: P35626 Human | P26819 Rat
Alternative names: ADRBK2 Adrenergic, beta, receptor kinase 2 ARBK2_HUMAN BARK2 Beta adrenergic receptor kinase 2 Beta ARK 2 Beta-adrenergic receptor kinase 2 Beta-ARK-2 EC 2.7.11.15 G protein coupled receptor kinase 3 G-protein-coupled receptor kinase 3 GRK3
Images
HA500317_1.jpg Fig1: Western blot analysis of GRK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500317, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: SHSY5Y cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Rat stomachl tissue lysate

Predicted band size: 80 kDa
Observed band size: 75 kDa
HA500317_2.jpg Fig2: ICC staining of GRK3 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500317, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500317_3.jpg Fig3: ICC staining of GRK3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500317, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500317_4.jpg Fig4: Flow cytometric analysis of GRK3 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500317, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.