GRK3 Rabbit Polyclonal Antibody
cat.: HA500317
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GRK3 aa 450-688 / 688. |
| Positive control: | Jurkat cell lysate, SHSY5Y cell lysate, HL-60 cell lysate, rat stomachl tissue lysate, MG-63, SiHa, SH-SY5Y. |
| Subcellular location: | cytosol, plasma membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50-1:200 1:500-1,000 |
| Uniprot #: | SwissProt: P35626 Human | P26819 Rat |
| Alternative names: | ADRBK2 Adrenergic, beta, receptor kinase 2 ARBK2_HUMAN BARK2 Beta adrenergic receptor kinase 2 Beta ARK 2 Beta-adrenergic receptor kinase 2 Beta-ARK-2 EC 2.7.11.15 G protein coupled receptor kinase 3 G-protein-coupled receptor kinase 3 GRK3 |
Images
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Fig1:
Western blot analysis of GRK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500317, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: SHSY5Y cell lysate Lane 3: HL-60 cell lysate Lane 4: Rat stomachl tissue lysate Predicted band size: 80 kDa Observed band size: 75 kDa |
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Fig2: ICC staining of GRK3 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500317, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of GRK3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500317, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Flow cytometric analysis of GRK3 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500317, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.