CNTFR Rabbit Polyclonal Antibody
cat.: HA500319
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CNTFR aa 1-50. |
| Positive control: | Mouse brain tissue lysate, mouse cerebellum tissue lysate, human skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, SH-SY5Y, mouse heart tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:200 1:200 |
| Uniprot #: | SwissProt: P26992 Human | O88507 Mouse | Q08406 Rat |
| Alternative names: | Ciliary neurotrophic factor receptor alpha Ciliary neurotrophic factor receptor Ciliary neurotrophic factor receptor subunit alpha CNTF Receptor alpha CNTF receptor subunit alpha CNTFR Alpha CNTFR CNTFR-alpha CNTFR_HUMAN |
Images
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Fig1:
Western blot analysis of CNTFR on different lysates with Rabbit anti-CNTFR antibody (HA500319) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Human skeletal muscle tissue lysate Lane 4: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 70/65/35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500319) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling CNTFR with Rabbit anti-CNTFR antibody (HA500319) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CNTFR antibody (HA500319) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CNTFR antibody (HA500319) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500319) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.