IL-12RB1 Rabbit Polyclonal Antibody
cat.: HA500321
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human IL-12RB1 aa 51-100.
Positive control: 293T cell lysates, human placenta tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: P42701 Human
Alternative names: CD212 cluster of differentiation 212 I12R1_HUMAN IL-12 receptor beta component IL-12 receptor subunit beta-1 IL-12R subunit beta-1 IL-12R-beta-1 IL-12RB1 IL12 receptor beta component IL12RB Il12rb1 IMD3 IMD30 Interleukin 12 receptor beta 1 chain Interleukin 12 receptor subunit beta 1 Interleukin-12 receptor subunit beta-1 MGC34454
Images
HA500321_1.jpg Fig1: Western blot analysis of IL-12RB1 on 293T cell lysates with Rabbit anti-IL-12RB1 antibody (HA500321) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 73 kDa
Observed band size: 65 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500321) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.