| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-12RB1 aa 51-100. |
| Positive control: | 293T cell lysates, human placenta tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P42701 Human |
| Alternative names: | CD212 cluster of differentiation 212 I12R1_HUMAN IL-12 receptor beta component IL-12 receptor subunit beta-1 IL-12R subunit beta-1 IL-12R-beta-1 IL-12RB1 IL12 receptor beta component IL12RB Il12rb1 IMD3 IMD30 Interleukin 12 receptor beta 1 chain Interleukin 12 receptor subunit beta 1 Interleukin-12 receptor subunit beta-1 MGC34454 |
|
Fig1:
Western blot analysis of IL-12RB1 on 293T cell lysates with Rabbit anti-IL-12RB1 antibody (HA500321) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 73 kDa Observed band size: 65 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500321) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |