IL-10RB Rabbit Polyclonal Antibody
cat.: HA500322
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-10RB aa 170-220 (Extracellular domain). |
| Positive control: | 293T cell lysate, Jurkat cell lysate, Hela cell lysate, SiHa, THP-1. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:200 1:500-1,000 |
| Uniprot #: | SwissProt: Q08334 Human |
| Alternative names: | Cd210 CDw210b CRF2 4 CRF2-4 CRFB4 Cytokine receptor class II Cytokine receptor class II CRF2 4 Cytokine receptor class-II member 4 Cytokine receptor family 2 member 4 Cytokine receptor family II member 4 D21S58 D21S66 I10R2_HUMAN IL-10 receptor subunit beta IL-10R subunit 2 IL-10R subunit beta IL-10R2 IL-10RB IL10R2 IL10RB Interleukin 10 receptor beta Interleukin-10 receptor subunit 2 Interleukin-10 receptor subunit beta |
Images
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Fig1:
Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA500322) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 42 kDa (Glycoprotein) Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500322) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of SiHa cells labeling IL-10RB with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of THP-1 cells labeling IL-10RB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500322, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.