AE 1 Rabbit Polyclonal Antibody
cat.: HA500323
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AE 1 aa 862-911. |
| Positive control: | 293T cell lysate, 293 cell lysate, human kidney tissue, K562. |
| Subcellular location: | Cell membrane, Basolateral cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:600 1:500-1:1,000 |
| Uniprot #: | SwissProt: P02730 Human | P04919 Mouse |
| Alternative names: | AE 1 AE1 Anion exchange protein 1 Anion exchanger 1 B3AT_HUMAN Band 3 anion transport protein Band 3 BND3 CD233 DI Diego blood group EMPB3 EPB3 Erythrocyte membrane protein band 3 Erythroid anion exchange protein FR Froese blood group RTA1A SLC4A1 Solute carrier family 4 anion exchanger member 1 Solute carrier family 4 member 1 SW Swann blood group Waldner blood group WD WD1 WR Wright blood group |
Images
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Fig1:
Western blot analysis of AE 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500323, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: 293 cell lysate Predicted band size: 102 kDa Observed band size: 85/48 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AE 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500323, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of K562 cells labeling AE 1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500323, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.