WSX-1 Rabbit Polyclonal Antibody
cat.: HA500324
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human WSX-1 aa 71-120. |
| Positive control: | A549 cell lysate, Hela cell lysate, LNCAP cell lysates, K562. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB FC |
1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q6UWB1 Human |
| Alternative names: | Class I cytokine receptor CRL1 Cytokine receptor-like 1 I27RA_HUMAN IL-27 receptor subunit alpha IL-27R subunit alpha IL-27R-alpha IL-27RA IL27R alpha IL27R Il27ra Interleukin 27 receptor alpha chain precursor Interleukin 27 receptor Interleukin-27 receptor subunit alpha Protein CRL1 T cell cytokine receptor type 1 TCCR Type 1 T cell cytokine receptor Type I T-cell cytokine receptor WSX-1 WSX1 ZcytoR1 |
Images
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Fig1:
Western blot analysis of WSX-1 on different lysates with Rabbit anti-WSX-1 antibody (HA500324) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 74 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500324) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of WSX-1 on LNCAP cell lysates with Rabbit anti-WSX-1 antibody (HA500324) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500324) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Flow cytometric analysis of K562 cells labeling WSX-1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500324, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.