Cyclin D2 Rabbit Polyclonal Antibody
cat.: HA500325
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cyclin D2 aa 30-79. |
| Positive control: | Mouse spleen tissue lysate, Hela cell lysate, rat spleen tissue lysates. |
| Subcellular location: | Nucleus, Cytoplasm, Membrane. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P30279 Human | P30280 Mouse | Q04827 Rat |
| Alternative names: | CCND 2 ccnd2 CCND2_HUMAN CyclinD2 G1/S specific cyclin D2 G1/S-specific cyclin-D2 KIAK0002 MGC102758 MPPH3 |
Images
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Fig1:
Western blot analysis of Cyclin D2 on different lysates with Rabbit anti-Cyclin D2 antibody (HA500325) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate, 20 µg/Lane Lane 2: Hela cell lysate, 10 µg/Lane Predicted band size: 33 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500325) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Cyclin D2 on rat spleen tissue lysates with Rabbit anti-Cyclin D2 antibody (HA500325) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500325) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.