Cyclin D2 Rabbit Polyclonal Antibody
cat.: HA500325
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cyclin D2 aa 1-289. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Nucleus membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P30279 Human | P30280 Mouse | Q04827 Rat |
| Alternative names: | CCND 2 ccnd2 CCND2_HUMAN CyclinD2 G1/S specific cyclin D2 G1/S-specific cyclin-D2 KIAK0002 MGC102758 MPPH3 |
Images
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Fig1:
Western blot analysis of Cyclin D2 on different lysates with Rabbit anti-Cyclin D2 antibody (HA500325) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: Caco-2 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500325) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.