PERK Rabbit Polyclonal Antibody
cat.: HA500326
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 125 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PERK aa 1,067-1,116/1,116.
Positive control: MCF-7 cell lysate, 293 cell lysate, HepG2 cell lysate, Hela cell lysate.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
1:1,000
Uniprot #: SwissProt: Q9NZJ5 Human
Alternative names: DKFZp781H1925 E2AK3_HUMAN EC 2.7.11.1 Eif2ak3 Eukaryotic translation initiation factor 2 alpha kinase 3 Eukaryotic translation initiation factor 2-alpha kinase 3 Heme regulated EIF2 alpha kinase HRI HsPEK Pancreatic eIF2 alpha kinase Pancreatic eIF2-alpha kinase PEK PRKR like endoplasmic reticulum kinase PRKR-like endoplasmic reticulum kinase WRS
Images
HA500326_1.jpg Fig1: Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (HA500326) at 1/1,000 dilution.

Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 125 kDa
Observed band size: 130 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500326) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.