PERK Rabbit Polyclonal Antibody
cat.: HA500326
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 125 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PERK aa 1,067-1,116/1,116. |
| Positive control: | THP-1 cell lysate, HEK-293 cell lysate, K-562 cell lysate |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9NZJ5 Human |
| Alternative names: | DKFZp781H1925 E2AK3_HUMAN EC 2.7.11.1 Eif2ak3 Eukaryotic translation initiation factor 2 alpha kinase 3 Eukaryotic translation initiation factor 2-alpha kinase 3 Heme regulated EIF2 alpha kinase HRI HsPEK Pancreatic eIF2 alpha kinase Pancreatic eIF2-alpha kinase PEK PRKR like endoplasmic reticulum kinase PRKR-like endoplasmic reticulum kinase WRS |
Images
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Fig1:
Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (HA500326) at 1/1,000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 11 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500326, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 125.2 kDa Observed band size: 125 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.