GRB7 Rabbit Polyclonal Antibody
cat.: HA500328
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 60 kDa
Isotype: IgG
Immunogen: Recombinant protein within human GRB7 aa 1-200/532.
Positive control: SK-Br-3 cell lysates.
Subcellular location: Cell membrane, Cytoplasm, Focal adhesion, Cytoplasmic granule, Cell projection.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: Q14451 Human
Alternative names: B47 Epidermal growth factor receptor GRB 7 Epidermal growth factor receptor GRB-7 GRB7 adapter protein GRB7 GRB7_HUMAN Growth factor receptor bound protein 7 Growth factor receptor-bound protein 7 OTTHUMP00000164349 OTTHUMP00000164350 OTTHUMP00000164351 OTTHUMP00000164352
Images
HA500328_1.jpg Fig1: Western blot analysis of GRB7 on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500328, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Western blot analysis of GRB7 on SK-Br-3 cell lysates with Rabbit anti-GRB7 antibody (HA500328) at 1/2,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 60 kDa
Observed band size: 60 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500328) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.