GRB7 Rabbit Polyclonal Antibody
cat.: HA500328
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GRB7 aa 1-200/532. |
| Positive control: | SK-Br-3 cell lysates. |
| Subcellular location: | Cell membrane, Cytoplasm, Focal adhesion, Cytoplasmic granule, Cell projection. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q14451 Human |
| Alternative names: | B47 Epidermal growth factor receptor GRB 7 Epidermal growth factor receptor GRB-7 GRB7 adapter protein GRB7 GRB7_HUMAN Growth factor receptor bound protein 7 Growth factor receptor-bound protein 7 OTTHUMP00000164349 OTTHUMP00000164350 OTTHUMP00000164351 OTTHUMP00000164352 |
Images
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Fig1:
Western blot analysis of GRB7 on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500328, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Western blot analysis of GRB7 on SK-Br-3 cell lysates with Rabbit anti-GRB7 antibody (HA500328) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500328) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.