Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 53 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human Bcl2-L-13 aa 151-350/485. |
Positive control: | Hela cell lysate, Jurkat cell lysate, Raji cell lysate. |
Subcellular location: | Mitochondrion membrane, Nucleus. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q9BXK5 Human |
Alternative names: | Apoptosis facilitator B2L13_HUMAN Bcl 2 like 13 Bcl 2 like 13 protein Bcl rambo Bcl-2-like protein 13 Bcl-rambo BCL2 like 13 apoptosis facilitator BCL2 like 13 Bcl2 like 13 protein Bcl2-L-13 BCL2L13 MIL 1 MIL1 MIL1 protein Protein Mil1 |
Fig1:
Western blot analysis of Bcl2-L-13 on different lysates with Rabbit anti-Bcl2-L-13 antibody (HA500329) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500329) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |