| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Bcl2-L-13 aa 151-350/485. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, Raji cell lysate. |
| Subcellular location: | Mitochondrion membrane, Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q9BXK5 Human |
| Alternative names: | Apoptosis facilitator B2L13_HUMAN Bcl 2 like 13 Bcl 2 like 13 protein Bcl rambo Bcl-2-like protein 13 Bcl-rambo BCL2 like 13 apoptosis facilitator BCL2 like 13 Bcl2 like 13 protein Bcl2-L-13 BCL2L13 MIL 1 MIL1 MIL1 protein Protein Mil1 |
|
Fig1:
Western blot analysis of Bcl2-L-13 on HeLa (Human cervical adenocarcinoma cell) lysates with Rabbit anti-Bcl2-L-13 antibody (HA500329) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500329, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52.7 kDa Observed band size: 85 kDa |