Phospho-RIP3 (T231 + S232) Rabbit Polyclonal Antibody
cat.: HA500330
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse RIP3 aa 201-250/518. |
| Positive control: | L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 2 hours, L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 3.5 hours. |
| Subcellular location: | Cytosol, Nucleus. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q9QZL0 Mouse |
| Alternative names: | Receptor interacting protein 3 Receptor interacting serine threonine kinase 3 Receptor interacting serine/threonine protein kinase 3 Receptor-interacting protein 3 Receptor-interacting serine/threonine-protein kinase 3 RIP 3 RIP like protein kinase 3 RIP-3 RIP-like protein kinase 3 RIPK 3 RIPK3 RIPK3_HUMAN |
Images
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Fig1:
Western blot analysis of Phospho-RIP3 (T231 + S232) on different lysates with Rabbit anti-Phospho-RIP3 (T231 + S232) antibody (HA500330) at 1/500 dilution. Lane 1: L929 cell lysate Lane 2: L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 2 hours Lane 3: L929 cell lysate treated with 20 µM Z-VAD and 20 ng/mL mTNF-α for 3.5 hours Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500330) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.