MED12 Rabbit Polyclonal Antibody
cat.: HA500331
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 243 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MED12 aa 1250-1450. |
| Positive control: | Hela cell lysate, K562 cell lysate, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200 |
| Uniprot #: | SwissProt: Q93074 Human |
| Alternative names: | Activator recruited cofactor 240 kDa component Activator-recruited cofactor 240 kDa component ARC240 CAG repeat protein 45 CAGH45 HOPA KIAA0192 MED12 MED12_HUMAN Mediator complex subunit 12 Mediator of RNA polymerase II transcription subunit 12 OPA containing protein OPA-containing protein Thyroid hormone receptor associated protein complex 230 kDa component Thyroid hormone receptor-associated protein complex 230 kDa component TNRC11 Trap230 Trinucleotide repeat containing gene 11 protein Trinucleotide repeat-containing gene 11 protein |
Images
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Fig1:
Western blot analysis of MED12 on different lysates with Rabbit anti-MED12 antibody (HA500331) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 243 kDa Observed band size: 243 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500331) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-MED12 antibody (HA500331) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500331) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.