TNFR2 Rabbit Polyclonal Antibody
cat.: HA500332
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TNFR2 aa 20-461 / 461. |
| Positive control: | Rat epididymis tissue, human uterus tissue, mouse testis tissue, HepG2. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
IHC-P FC |
1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P20333 Human | P25119 Mouse | Q80WY6 Rat |
| Alternative names: | CD120b p75 p75 TNF receptor p75TNFR p80 TNF alpha receptor p80 TNF-alpha receptor Soluble TNFR1B variant 1 TBP-2 TBPII TNF R II TNF R2 TNF R75 TNF-R2 TNF-RII TNFBR TNFR-II TNFR1B TNFR2 TNFR80 TNFRII Tnfrsf1b TNR1B_HUMAN Tumor necrosis factor beta receptor Tumor necrosis factor binding protein 2 Tumor necrosis factor receptor 2 Tumor necrosis factor receptor superfamily member 1B Tumor necrosis factor receptor type II Tumor necrosis factor-binding protein 2 |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-TNFR2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TNFR2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TNFR2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500332, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Flow cytometric analysis of TNFR2 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500332, 1/50) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.