TRPV6 Rabbit Polyclonal Antibody
cat.: HA500333
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 87 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to C terminal of Human TRPV6 .
Positive control: SW480 cell lysate, SK-Br-3 cell lysate, HepG2, HT-29, rat testis tissue, human kidney tissue, mouse colon tissue, human placenta tissue.
Subcellular location: Cell membrane, Membrane
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9H1D0 Human | Q91WD2 Mouse | Q9R186 Rat
Alternative names: ABP/ZF ABPZF Alu binding protein with zinc finger domain Calcium channel CaT1 Calcium transport protein 1 CaT L CaT Like CaT-L CaT-like CaT1 ecac ECaC2 Epithelial apical membrane calcium transporter/channel CaT1 Epithelial calcium channel 2 Epithelial calcium channel HSA277909 LP6728 MGC162545 NUDC Transient receptor potential cation channel subfamily V member 6 TrpV6 TRPV6_HUMAN ZFAB
Images
HA500333_1.jpg Fig1: Western blot analysis of TRPV6 on different lysates with Rabbit anti-TRPV6 antibody (HA500333) at 1/500 dilution.

Lane 1: SW480 cell lysate
Lane 2: SK-Br-3 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 87 kDa
Observed band size: 42 kDa (unglycosylated monomeric form of TRPV6)

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500333) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500333_2.jpg Fig2: ICC staining of TRPV6 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500333, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
HA500333_3.jpg Fig3: ICC staining of TRPV6 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500333, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
HA500333_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-TRPV6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500333, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500333_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRPV6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500333, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500333_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRPV6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500333, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500333_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-TRPV6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500333, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500333_8.jpg Fig8: Flow cytometric analysis of TRPV6 was done on HT-29 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500333, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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