Calcineurin B Rabbit Polyclonal Antibody
cat.: HA500346
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Calcineurin B aa 1-170 / 170. |
| Positive control: | Rat brain tissue lysate, mouse brain tissue lysate, SiHa, rat brain tissue, HepG2. |
| Subcellular location: | Cell membrane, sarcolemma, cell membrane, cytosol. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:200 1:600 1:500-1:1,000 |
| Uniprot #: | SwissProt: P63098 Human | Q63810 Mouse | P63100 Rat |
| Alternative names: | alpha isoform (calcineurin B, type I) calcineurin B, type I (19kDa) Calcineurin subunit B type 1 CALNB1 CANB1_HUMAN Cna2 CNB CNB1 OTTHUMP00000201960 OTTHUMP00000201961 Ppp3r1 PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform protein phosphatase3 (formerly2B), regulatory subunit B, alpha isoform Protein phosphatase 2B regulatory subunit 1 Protein phosphatase 2B regulatory subunit B alpha protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) Protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I) Protein phosphatase 3 regulatory subunit B alpha Protein phosphatase 3 regulatory subunit B alpha isoform 1 |
Images
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Fig1:
Western blot analysis of Calcineurin B on different lysates with Rabbit anti-Calcineurin B antibody (HA500346) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 15 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500346) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SiHa cells labeling Calcineurin B with Rabbit anti-Calcineurin B antibody (HA500346) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Calcineurin B antibody (HA500346) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Calcineurin B antibody (HA500346) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500346) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of HepG2 cells labeling Calcineurin B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500346, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.