TMED5 Rabbit Polyclonal Antibody
cat.: HA500347
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TMED5 aa 1-200 / 229. |
| Positive control: | Rat liver tissue lysate, A431 cell lysate, Jurkat cell lysate, Hela cell lysate, SiHa cell lysate, rat bladder tissue, mouse large intestine tissue. |
| Subcellular location: | Cis-Golgi network membrane, endoplasmic reticulum membrane, endoplasmic reticulum-Golgi intermediate compartment membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:600-1:5,000 |
| Uniprot #: | SwissProt: Q9Y3A6 Human | Q9CXE7 Mouse | Q6AXN3 Rat |
| Alternative names: | CGI 100 DKFZp781B2248 p24 family protein gamma-2 p24gamma2 p28 RP5 976O13.2 TMED 5 TMED5 TMED5_HUMAN Transmembrane emp24 domain containing protein 5 Transmembrane emp24 domain-containing protein 5 Transmembrane emp24 protein transport domain containing 5 UNQ397/PRO733 |
Images
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Fig1:
Western blot analysis of TMED5 on different lysates with Rabbit anti-TMED5 antibody (HA500347) at 1/500 dilution. Lane 1: Rat liver tissue lysate (20 µg/Lane) Lane 2: A431 cell lysate Lane 3: Jurkat cell lysate Lane 4: Hela cell lysate Lane 5: SiHa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 55 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500347) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.