| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RYBP aa 41-228 / 228. |
| Positive control: | SH-SY5Y cell lysate, mouse brain tissue lysate, rat kidney tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, nucleus, nucleoplasm. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q8N488 Human | Q8CCI5 Mouse Entrez Gene: 312603 Rat |
| Alternative names: | 2410018J24Rik AAP 1 AAP1 APAP 1 APAP-1 APAP1 Apoptin associating protein 1 Apoptin-associating protein 1 cb337 Death effector domain associated factor Death effector domain-associated factor Death effector domain-associated factor B DED associated factor DED-associated factor DED-associated factor B DEDAF MGC62213 RING1 and YY1 binding protein a RING1 and YY1 binding protein RING1 and YY1-binding protein RING1 and YY1-binding protein B Ring1 interactor RYBP RYBP RYBP_HUMAN rybpa wu:fi34f03 YEAF 1 YEAF1 YY1 and E4TF1 associated factor 1 YY1 and E4TF1-associated factor 1 YY1- and E4TF1/GABP-associated factor 1 zgc:153842 |
|
Fig1:
Western blot analysis of RYBP on NCCIT cell lysate with Rabbit anti-RYBP antibody (HA500348) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500348, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 24.8 kDa Observed band size: 30 kDa |