RYBP Rabbit Polyclonal Antibody
cat.: HA500348
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 25 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RYBP aa 1-200 / 228.
Positive control: SH-SY5Y cell lysate, mouse brain tissue lysate, rat kidney tissue lysate, rat brain tissue lysate.
Subcellular location: Cytoplasm, nucleus, nucleoplasm.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q8N488 Human | Q8CCI5 Mouse
Entrez Gene: 312603 Rat
Alternative names: 2410018J24Rik AAP 1 AAP1 APAP 1 APAP-1 APAP1 Apoptin associating protein 1 Apoptin-associating protein 1 cb337 Death effector domain associated factor Death effector domain-associated factor Death effector domain-associated factor B DED associated factor DED-associated factor DED-associated factor B DEDAF MGC62213 RING1 and YY1 binding protein a RING1 and YY1 binding protein RING1 and YY1-binding protein RING1 and YY1-binding protein B Ring1 interactor RYBP RYBP RYBP_HUMAN rybpa wu:fi34f03 YEAF 1 YEAF1 YY1 and E4TF1 associated factor 1 YY1 and E4TF1-associated factor 1 YY1- and E4TF1/GABP-associated factor 1 zgc:153842
Images
HA500348_1.jpg Fig1: Western blot analysis of RYBP on different lysates with Rabbit anti-RYBP antibody (HA500348) at 1/500 dilution.

Lane 1: SH-SY5Y cell lysate, 10 µg/Lane
Lane 2: Mouse brain tissue lysate, 20 µg/Lane
Lane 3: Rat kidney tissue lysate, 20 µg/Lane
Lane 4: Rat brain tissue lysate, 20 µg/Lane

Predicted band size: 25 kDa
Observed band size: 25 kDa

Exposure time: 30 seconds;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500348) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.