Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 25 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human RYBP aa 1-200 / 228. |
Positive control: | SH-SY5Y cell lysate, mouse brain tissue lysate, rat kidney tissue lysate, rat brain tissue lysate. |
Subcellular location: | Cytoplasm, nucleus, nucleoplasm. |
Recommended Dilutions:
WB |
1:500 |
Uniprot #: | SwissProt: Q8N488 Human | Q8CCI5 Mouse Entrez Gene: 312603 Rat |
Alternative names: | 2410018J24Rik AAP 1 AAP1 APAP 1 APAP-1 APAP1 Apoptin associating protein 1 Apoptin-associating protein 1 cb337 Death effector domain associated factor Death effector domain-associated factor Death effector domain-associated factor B DED associated factor DED-associated factor DED-associated factor B DEDAF MGC62213 RING1 and YY1 binding protein a RING1 and YY1 binding protein RING1 and YY1-binding protein RING1 and YY1-binding protein B Ring1 interactor RYBP RYBP RYBP_HUMAN rybpa wu:fi34f03 YEAF 1 YEAF1 YY1 and E4TF1 associated factor 1 YY1 and E4TF1-associated factor 1 YY1- and E4TF1/GABP-associated factor 1 zgc:153842 |
Fig1:
Western blot analysis of RYBP on different lysates with Rabbit anti-RYBP antibody (HA500348) at 1/500 dilution. Lane 1: SH-SY5Y cell lysate, 10 µg/Lane Lane 2: Mouse brain tissue lysate, 20 µg/Lane Lane 3: Rat kidney tissue lysate, 20 µg/Lane Lane 4: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500348) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |