YTHDF1 Rabbit Polyclonal Antibody
cat.: HA500350
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Full length of Human YTHDF1. |
| Positive control: | MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm, P-body. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:100 1:2,000 |
| Uniprot #: | SwissProt: Q9BYJ9 Human | P59326 Mouse Entrez Gene: 296467 Rat |
| Alternative names: | C20orf21 DACA 1 DACA-1 Dermatomyositis associated with cancer putative autoantigen 1 YTH domain family 1 YTH domain family member 1 YTH domain family protein 1 YTHD1 YTHD1_HUMAN Ythdf1 |
Images
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Fig1:
Western blot analysis of YTHDF1 on different lysates with Rabbit anti-YTHDF1 antibody (HA500350) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of YTHDF1 on NIH/3T3 cell lysates with Rabbit anti-YTHDF1 antibody (HA500350) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500350) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling YTHDF1 with Rabbit anti-YTHDF1 antibody (HA500350) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-YTHDF1 antibody (HA500350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-YTHDF1 antibody (HA500350) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500350) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-YTHDF1 antibody (HA500350) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500350) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.