YTHDF2 Rabbit Polyclonal Antibody
cat.: HA500351
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Full length of Human YTHDF2. |
| Positive control: | 293T cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3. |
| Subcellular location: | Nucleus, cytosol. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:100 |
| Uniprot #: | SwissProt: Q9Y5A9 Human | Q91YT7 Mouse |
| Alternative names: | 9430020E02Rik CAHL CLL associated antigen KW 14 CLL-associated antigen KW-14 HGRG8 High glucose regulated protein 8 High-glucose-regulated protein 8 NY REN 2 Renal carcinoma antigen NY REN 2 Renal carcinoma antigen NY-REN-2 YTH domain family 2 YTH domain family member 2 YTH domain family protein 2 YTH N6 methyladenosine RNA binding protein 2 YTHD2_HUMAN YTHDF 2 YTHDF2 |
Images
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Fig1:
Western blot analysis of YTHDF2 on different lysates with Rabbit anti-YTHDF2 antibody (HA500351) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of YTHDF2 on NIH/3T3 cell lysates with Rabbit anti-YTHDF2 antibody (HA500351) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500351) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling YTHDF2 with Rabbit anti-YTHDF2 antibody (HA500351) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-YTHDF2 antibody (HA500351) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.