PSMC5 Rabbit Polyclonal Antibody
cat.: HA500352
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PSMC5 aa 205-406 / 406. |
| Positive control: | A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse heart tissue lysate, Rat testis tissue lysate, Rat kidney tissue lysate, rat brain tissue, human colon tissue, human skin tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,500 |
| Uniprot #: | SwissProt: P62195 Human | P62196 Mouse | P62198 Rat |
| Alternative names: | 26S protease regulatory subunit 8 26S proteasome AAA-ATPase subunit RPT6 Cim3 MSUG1 protein p45 p45/SUG Proteasome 26S ATPase subunit 5 Proteasome 26S subunit ATPase 5 Proteasome prosome macropain 26S subunit ATPase 5 Proteasome subunit p45 PRS8_HUMAN PSMC5 Rpt6 S8 SUG1 Tat binding protein homolog 10 TBP10 Thyroid hormone receptor interacting protein 1 Thyroid hormone receptor-interacting protein 1 Thyroid receptor interactor 1 TRIP1 TRIP1(SUG1) |
Images
|
Fig1:
Western blot analysis of PSMC5 on different lysates with Rabbit anti-PSMC5 antibody (HA500352) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Mouse heart tissue lysate Lane 5: Rat testis tissue lysate Lane 6: Rat kidney tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time:6 seconds; ECL: K1801 Negative expression of PSMC5 protein in Daudi cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500352, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 46 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PSMC5 antibody (HA500352) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500352) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.