HA500353

PSMD2 Rabbit Polyclonal Antibody

cat.: HA500353

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 100 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human PSMD2 aa 1-200 / 908.
Positive control:	293T cell lysate, Jurkat cell lysate, Hela cell lysate, NIH/3T3 cell lysate, mouse heart tissue lysate, rat testis tissue lysate, rat heart tissue lysate, SiHa, rat large intestine tissue, human lung carcinoma tissue, human striated muscle tissue, mouse heart tissue.
Subcellular location:	Cytosol, extracellular exosome, extracellular region, nucleoplasm, nucleus, proteasome storage granule.
Recommended Dilutions: WB IF-Cell IHC-P	1:1,000 1-200 1-1,000-1-1,500
Uniprot #:	SwissProt: Q13200 Human \| Q8VDM4 Mouse \| Q4FZT9 Rat
Alternative names:	26S proteasome non-ATPase regulatory subunit 2 26S proteasome regulatory subunit RPN1 26S proteasome regulatory subunit S2 26S proteasome subunit p97 55.11 protein MGC14274 P97 Proteasome (prosome macropain) 26S subunit non ATPase 2 Proteasome 26S subunit, non-ATPase, 2 Proteasome 26S, subunit 2 Protein 55.11 Psmd2 PSMD2_HUMAN Rpn1 S2 TNFR associated protein 2 TRAP2 Tumor necrosis factor receptor associated protein 2 Tumor necrosis factor type 1 receptor-associated protein 2

Images

Fig1: Western blot analysis of PSMD2 on different lysates with Rabbit anti-PSMD2 antibody (HA500353) at 1/1,000 dilution.

Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Hela cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (27 µg/Lane)
Lane 6: Rat testis tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (20 µg/Lane)

Predicted band size: 100 kDa
Observed band size: 90 kDa

Exposure time: 29 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500353) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Fig2: Immunocytochemistry analysis of SiHa cells labeling PSMD2 with Rabbit anti-PSMD2 antibody (HA500353) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD2 antibody (HA500353) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

	Fig3: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-PSMD2 antibody (HA500353) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500353) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PSMD2 antibody (HA500353) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500353) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-PSMD2 antibody (HA500353) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500353) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-PSMD2 antibody (HA500353) at 1/1,500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500353) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.