PSMD7 Rabbit Polyclonal Antibody
cat.: HA500354
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PSMD7 aa 1-200 / 324.
Positive control: HeLa cell lysate, K-562 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human colon tissue, mouse testis tissue, rat brain tissue, rat testis tissue, HeLa.
Subcellular location: Cytosol, extracellular exosome, extracellular region, nucleoplasm, nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:1,000
1:200-1:600
1:100
1:1,000
Uniprot #: SwissProt: P51665 Human | P26516 Mouse
Entrez Gene: 307821 Rat
Alternative names: 26S proteasome non ATPase regulatory subunit 7 26S proteasome non-ATPase regulatory subunit 7 26S proteasome regulatory subunit RPN8 26S proteasome regulatory subunit S12 integration site gene, mouse, homolog of Moloney leukemia virus 34 proviral Moloney leukemia virus 34 proviral integration MOV34 Mov34 homolog Mov34 protein homolog MOV34L P40 Proteasome (prosome, macropain) 26S subunit, non ATPase 7 Proteasome (prosome, macropain) 26S subunit, non ATPase, 7 (Mov34 homolog) Proteasome 26S S12 Proteasome 26S subunit, no-ATPase, 7 Proteasome subunit p40 PSD7_HUMAN PSMD 7 PSMD7 Rpn8 S12
Images
HA500354_1.jpg Fig1: Western blot analysis of PSMD7 on different lysates with Rabbit anti-PSMD7 antibody (HA500354) at 1/500 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (16 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (12 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse testis tissue lysate (20 µg/Lane)
Lane 7: Rat testis tissue lysate (20 µg/Lane)

Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500354) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
HA500354_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMD7 antibody (HA500354) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500354_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PSMD7 antibody (HA500354) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500354_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMD7 antibody (HA500354) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500354_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PSMD7 antibody (HA500354) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500354_6.jpg Fig6: Immunocytochemistry analysis of HeLa cells labeling PSMD7 with Rabbit anti-PSMD7 antibody (HA500354) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PSMD7 antibody (HA500354) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA500354_7.jpg Fig7: Flow cytometric analysis of HeLa cells labeling PSMD7.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500354, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.