PSMD7 Rabbit Polyclonal Antibody
cat.: HA500354
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PSMD7 aa 1-200 / 324. |
| Positive control: | K562 cell lysate, mouse testis tissue lysate, Hela cell lysate, rat testis tissue lysate, rat brain tissue, human colon tissue. |
| Subcellular location: | Cytosol, extracellular exosome, extracellular region, nucleoplasm, nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200-1:600 |
| Uniprot #: | SwissProt: P51665 Human | P26516 Mouse Entrez Gene: 307821 Rat |
| Alternative names: | 26S proteasome non ATPase regulatory subunit 7 26S proteasome non-ATPase regulatory subunit 7 26S proteasome regulatory subunit RPN8 26S proteasome regulatory subunit S12 integration site gene, mouse, homolog of Moloney leukemia virus 34 proviral Moloney leukemia virus 34 proviral integration MOV34 Mov34 homolog Mov34 protein homolog MOV34L P40 Proteasome (prosome, macropain) 26S subunit, non ATPase 7 Proteasome (prosome, macropain) 26S subunit, non ATPase, 7 (Mov34 homolog) Proteasome 26S S12 Proteasome 26S subunit, no-ATPase, 7 Proteasome subunit p40 PSD7_HUMAN PSMD 7 PSMD7 Rpn8 S12 |
Images
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Fig1:
Western blot analysis of PSMD7 on different lysates with Rabbit anti-PSMD7 antibody (HA500354) at 1/500 dilution. Lane 1: K562 cell lysate (10 µg/Lane) Lane 2: Mouse testis tissue lysate (20 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500354) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PSMD7 on different lysates with Rabbit anti-PSMD7 antibody (HA500354) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500354) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMD7 antibody (HA500354) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500354) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMD7 antibody (HA500354) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500354) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.