TIP49A Rabbit Polyclonal Antibody
cat.: HA500355
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TIP49A aa 121-320 / 456. |
| Positive control: | Hela cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse skeletal muscle tissue lysate, rat skin tissue lysate, Rat skeletal muscle lysate, human liver tissue, human testis tissue, rat trachea tissue, human fallopian tube tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:400-1:800 |
| Uniprot #: | SwissProt: Q9Y265 Human | P60122 Mouse | P60123 Rat |
| Alternative names: | 49 kDa TATA box binding protein interacting protein 49 kDa TATA box-binding protein-interacting protein 49 kDa TBP interacting protein 49 kDa TBP-interacting protein 54 kDa erythrocyte cytosolic protein ECP-54 ECP54 ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD INO80 complex subunit H NMP 238 Nuclear matrix protein 238 Pontin 52 PONTIN RuvB like 1 RuvB like AAA ATPase 1 RuvB-like 1 RUVB1_HUMAN RUVBL1 RVB1 TAP54 alpha TAP54-alpha TIP49 TIP49a TIP60 associated protein 54 alpha TIP60-associated protein 54-alpha |
Images
|
Fig1:
Western blot analysis of TIP49A on different lysates with Rabbit anti-TIP49A antibody (HA500355) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: MCF-7 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 6: Rat skin tissue lysate (40 µg/Lane) Lane 7: Rat skeletal muscle lysate (24 µg/Lane) Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TIP49A antibody (HA500355) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500355) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TIP49A antibody (HA500355) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500355) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat trachea tissue with Rabbit anti-TIP49A antibody (HA500355) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500355) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-TIP49A antibody (HA500355) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500355) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-TIP49A antibody (HA500355) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500355) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.