Wnt10a Rabbit Polyclonal Antibody
cat.: HA500356
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Wnt10a aa 201-417 / 417. |
| Positive control: | RAW264.7 cell lysates. |
| Subcellular location: | Extracellular matrix, Secreted. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q9GZT5 Human | P70701 Mouse |
| Alternative names: | FLJ14301 OODD Protein Wnt-10a SSPS STHAG4 Wingless type MMTV integration site family member 10A WN10A_HUMAN wnt10a |
Images
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Fig1:
Western blot analysis of Wnt10a on RAW264.7 cell lysates with Rabbit anti-Wnt10a antibody (HA500356) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500356) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.