alpha 1 Spectrin Rabbit Polyclonal Antibody
cat.: HA500357
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 280 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human alpha 1 Spectrin aa 1-200 / 2,419. |
| Positive control: | K562 cell lysate, NIH/3T3 cell lysate, A549 cell lysate. |
| Subcellular location: | Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P02549 Human | P08032 Mouse |
| Alternative names: | Alpha I spectrin EL 2 EL2 Elliptocytosis 2 Elliptocytosis2 Erythrocyte alpha spectrin erythrocyte Erythroid alpha spectrin Erythroid alpha-spectrin Erythroid spectrin alpha HPP HS3 Spectrin alpha chain Spectrin alpha chain erythrocyte Spectrin alpha erythrocytic 1 SPH3 SPTA 1 SPTA SPTA1 SPTA1_HUMAN |
Images
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Fig1:
Western blot analysis of alpha 1 Spectrin on different lysates with Rabbit anti-alpha 1 Spectrin antibody (HA500357) at 1/1,000 dilution. Lane 1: K562 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 280 kDa Observed band size: 280 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500357) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.