Beta TRCP Rabbit Polyclonal Antibody
cat.: HA500358
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Beta TRCP aa 1-200 / 605. |
| Positive control: | HepG2 cell lysate, Hela cell lysate, 293T cell lysate, mouse testis tissue lysate, rat brain tissue lysate, mouse brain tissue lysate, A549, human colon tissue, mouse large intestine tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:100 1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q9Y297 Human | Q3ULA2 Mouse Entrez Gene: 361765 Rat |
| Alternative names: | Beta transducin repeat containing E3 ubiquitin protein ligase beta TrCP1 beta-transducin repeat containing BETA-TRCP betaTrCP btrC bTrCP bTrCP1 E3RSIkappaB F box and WD repeat protein 1B F box and WD repeats protein beta TrCP F-box and WD repeats protein beta-TrCP F-box/WD repeat protein 1A F-box/WD repeat-containing protein 1A FBW1A FBW1A_HUMAN FBXW1 FBXW1A FWD1 HOS mKIAA4123 pIkappaBalpha-E3 receptor subunit Slimb |
Images
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Fig1:
Western blot analysis of Beta TRCP on different lysates with Rabbit anti-Beta TRCP antibody (HA500358) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: Mouse testis tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 69 kDa Observed band size: 69/110 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500358) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A549 cells labeling Beta TRCP with Rabbit anti-Beta TRCP antibody (HA500358) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Beta TRCP antibody (HA500358) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Beta TRCP antibody (HA500358) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500358) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Beta TRCP antibody (HA500358) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500358) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.