PSMC6 Rabbit Polyclonal Antibody
cat.: HA500359
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PSMC6 aa 1-200 / 389.
Positive control: HepG2 cell lysate, PC-12 cell lysate, rat kidney tissue lysate, 293T cell lysate, SH-SY5Y cell lysate, mouse heart tissue lysate, mouse kidney tissue lysate, SH-SY5Y, rat hippocampus tissue, rat brain tissue, human kidney clear cell carcinoma tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000
1:100
1:1,000
Uniprot #: SwissProt: P62333 Human | P62334 Mouse
Entrez Gene: 289990 Rat
Alternative names: 26S protease regulatory subunit 10B 26S protease regulatory subunit S10B 26S proteasome AAA-ATPase subunit RPT4 CADP44 P44 Proteasome 26S subunit ATPase 6 Proteasome subunit p42 PRS10_HUMAN PSMC6 Rpt4 SUG2
Images
HA500359_1.jpg Fig1: Western blot analysis of PSMC6 on different lysates with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: PC-12 cell lysate (10 µg/Lane)
Lane 3: Rat kidney tissue lysate (20 µg/Lane)

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500359_2.jpg Fig2: Western blot analysis of PSMC6 on different lysates with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.

Lane 1: 293T cell lysate (10 µg/Lane)
Lane 2: SH-SY5Y cell lysate (10 µg/Lane)
Lane 3: Mouse heart tissue lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500359) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA500359_3.jpg Fig3: Immunocytochemistry analysis of SH-SY5Y cells labeling PSMC6 with Rabbit anti-PSMC6 antibody (HA500359) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMC6 antibody (HA500359) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA500359_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500359_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500359_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue with Rabbit anti-PSMC6 antibody (HA500359) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500359) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.