MPL Rabbit Polyclonal Antibody
cat.: HA500360
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MPL aa 551-600 / 635. |
| Positive control: | A549 cell lysate, Raji cell lysate, THP-1 cell lysate, SH-SY5Y, human placenta tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus, Cell surface. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:200 1:200 |
| Uniprot #: | SwissProt: P40238 Human |
| Alternative names: | C MPL CD110 mpl MPLV Myeloproliferative leukemia protein Myeloproliferative leukemia virus oncogene Proto-oncogene c-Mpl THCYT2 Thrombopoietin receptor TPO R TPO-R TPOR TPOR_HUMAN |
Images
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Fig1:
Western blot analysis of MPL on different lysates with Rabbit anti-MPL antibody (HA500360) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Raji cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 71 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500360) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling MPL with Rabbit anti-MPL antibody (HA500360) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MPL antibody (HA500360) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-MPL antibody (HA500360) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500360) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.