TPOR / MPL Rabbit Polyclonal Antibody
cat.: HA500360
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MPL aa 551-600 / 635. |
| Positive control: | A549 cell lysate, Raji cell lysate, THP-1 cell lysate, THP-1, SH-SY5Y. |
| Subcellular location: | Cell membrane, Golgi apparatus, Cell surface. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100-1:200 |
| Uniprot #: | SwissProt: P40238 Human |
| Alternative names: | C MPL CD110 mpl MPLV Myeloproliferative leukemia protein Myeloproliferative leukemia virus oncogene Proto-oncogene c-Mpl THCYT2 Thrombopoietin receptor TPO R TPO-R TPOR TPOR_HUMAN |
Images
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Fig1:
Western blot analysis of TPOR / MPL on different lysates with Rabbit anti-TPOR / MPL antibody (HA500360) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Raji cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 71 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500360) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of THP-1 cells labeling TPOR / MPL with Rabbit anti-TPOR / MPL antibody (HA500360) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TPOR / MPL antibody (HA500360) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of SH-SY5Y cells labeling TPOR / MPL with Rabbit anti-TPOR / MPL antibody (HA500360) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TPOR / MPL antibody (HA500360) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.