USP8 Rabbit Polyclonal Antibody
cat.: HA500362
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.3ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 128 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human USP8 aa 1-250. |
| Positive control: | A375 cell lysate, A431 cell lysate, HepG2 cell lysate, 293T cell lysate, MCF-7 cell lysate, mouse testis tissue lysate, SK-Br-3 cell lysate, F9 cell lysate, rat lung tissue lysate, Hela cell lysate, mouse kidney tissue, human colon carcinoma tissue. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P40818 Human | Q80U87 Mouse Entrez Gene: 296121 Rat |
| Alternative names: | Deubiquitinating enzyme 8 FLJ34456 hUBPy HumORF8 KIAA0055 MGC129718 SPG59 Ubiquitin carboxyl-terminal hydrolase 8 Ubiquitin isopeptidase Y ubiquitin specific peptidase 8 Ubiquitin thioesterase 8 Ubiquitin-specific-processing protease 8 UBP8_HUMAN UBPY Usp8 |
Images
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Fig1:
Western blot analysis of USP8 on different lysates with Rabbit anti-USP8 antibody (HA500362) at 1/500 dilution. Lane 1: A375 cell lysate (10 µg/Lane) Lane 2: A431 cell lysate (10 µg/Lane) Lane 3: HepG2 cell lysate (10 µg/Lane) Lane 4: 293T cell lysate (10 µg/Lane) Lane 5: MCF-7 cell lysate (10 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: SK-Br-3 cell lysate (10 µg/Lane) Lane 8: F9 cell lysate (10 µg/Lane) Lane 9: Rat lung tissue lysate (20 µg/Lane) Lane 10: Hela cell lysate (10 µg/Lane) Predicted band size: 128 kDa Observed band size: 128 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500362) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-USP8 antibody (HA500362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-USP8 antibody (HA500362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.