IFNGR2 Rabbit Polyclonal Antibody
cat.: HA500363
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.8ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human INGR2 22-71. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, A431 cell lysate, human skeletal muscle tissue lysates, SH-SY5Y. |
| Subcellular location: | Endoplasmic reticulum, Plasma membrane, Golgi apparatus. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:200 |
| Uniprot #: | SwissProt: P38484 Human Entrez Gene: 15980 Mouse | 360697 Rat |
| Alternative names: | Interferon gamma receptor 2Imported IFN-gamma receptor 2 IFN-gamma-R2 Interferon gamma receptor accessory factor 1 AF-1 Interferon gamma receptor beta-chain IFN-gamma-R-beta Interferon gamma transducer 1 IFNGR2 IFNGT1 |
Images
|
Fig1:
Western blot analysis of IFNGR2 on different lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of IFNGR2 on human skeletal muscle tissue lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of SH-SY5Y cells labeling IFNGR2 with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.