IFNGR2 Rabbit Polyclonal Antibody
cat.: HA500363
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.8ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human INGR2 22-71. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, A431 cell lysate, human skeletal muscle tissue lysates, SH-SY5Y. |
| Subcellular location: | Endoplasmic reticulum, Plasma membrane, Golgi apparatus. |
| Recommended Dilutions:
WB IF-Cell |
1:1000 1:200 |
| Uniprot #: | SwissProt: P38484 Human Entrez Gene: 15980 Mouse | 360697 Rat |
| Alternative names: | Interferon gamma receptor 2Imported IFN-gamma receptor 2 IFN-gamma-R2 Interferon gamma receptor accessory factor 1 AF-1 Interferon gamma receptor beta-chain IFN-gamma-R-beta Interferon gamma transducer 1 IFNGR2 IFNGT1 |
Images
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Fig1:
Western blot analysis of IFNGR2 on different lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: SH-SY5Y (Human neuroblastoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 59 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500363, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 38 kDa Observed band size: 40 kDa |
|
Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling IFNGR2 with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.