HA500364

Podoplanin Rabbit Polyclonal Antibody

cat.: HA500364

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IHC-P
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1.2ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 17 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human Podoplanin aa 23-72.
Positive control:	Human lung tissue lysate, Human placenta tissue lysate, human appendix tissue, human colon cancer tissue, human ovary tissue, human liver tissue.
Subcellular location:	Membrane.
Recommended Dilutions: WB IHC-P	1:1,000 1:500-1:1,000
Uniprot #:	SwissProt: Q86YL7 Human
Alternative names:	Aggrus Glycoprotein 36 KD Glycoprotein 36 gp 36 GP 38 GP 40 gp36 GP38 GP40 HT1A 1 HT1A1 hT1alpha 1 hT1alpha 2 hT1alpha1 hT1alpha2 Lung type I cell membrane associated glycoprotein Lung type I cell membrane associated glycoprotein isoform a Lung type I cell membrane associated glycoprotein T1A 2 OTS 8 OTS8 OTTHUMP00000009640 OTTHUMP00000044504 PA2.26 PA2.26 antigen Pdpn PDPN_HUMAN Podoplanin PSEC0003 PSEC0025 T1 alpha T1 ALPHA GENE T1-alpha T1A 2 T1A TI1A TIA 2 TIA2

Images

Fig1: Western blot analysis of Podoplanin on different lysates with Rabbit anti-Podoplanin antibody (HA500364) at 1/1,000 dilution.

Lane 1: Human lung tissue lysate, 20 µg/Lane.
Lane 2: Human placenta tissue lysate, 20 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 37 kDa

Exposure time: 67 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500364) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Podoplanin on different lysates with Rabbit anti-Podoplanin antibody (HA500364) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Podoplanin KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 37 kDa

Exposure time: 15 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500364) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500364) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500364) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500364) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500364) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500364) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.