Podoplanin Rabbit Polyclonal Antibody
cat.: HA500364
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Podoplanin aa 23-72. |
| Positive control: | Human ovary tissue, human liver tissue, human lung tissue lysate, human placenta tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: Q86YL7 Human |
| Alternative names: | Aggrus Glycoprotein 36 KD Glycoprotein 36 gp 36 GP 38 GP 40 gp36 GP38 GP40 HT1A 1 HT1A1 hT1alpha 1 hT1alpha 2 hT1alpha1 hT1alpha2 Lung type I cell membrane associated glycoprotein Lung type I cell membrane associated glycoprotein isoform a Lung type I cell membrane associated glycoprotein T1A 2 OTS 8 OTS8 OTTHUMP00000009640 OTTHUMP00000044504 PA2.26 PA2.26 antigen Pdpn PDPN_HUMAN Podoplanin PSEC0003 PSEC0025 T1 alpha T1 ALPHA GENE T1-alpha T1A 2 T1A TI1A TIA 2 TIA2 |
Images
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Fig1:
Western blot analysis of Podoplanin on different lysates with Rabbit anti-Podoplanin antibody (HA500364) at 1/1,000 dilution. Lane 1: Human lung tissue lysate, 20 µg/Lane. Lane 2: Human placenta tissue lysate, 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 37 kDa Exposure time: 67 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500364) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500364) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500364) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Podoplanin antibody (HA500364) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500364) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.