IL22 RA2 Rabbit Polyclonal Antibody
cat.: HA500365
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.6ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL22 RA2 aa 22-71. |
| Positive control: | Mouse lung tissue lysate, human lung tissue lysate, human kidney tissue, SiHa. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200 1:200 |
| Uniprot #: | SwissProt: Q969J5 Human | Q80XF5 Mouse |
| Alternative names: | IL 22BP IL 22RA2 IL22RA2 Class II cytokine receptor CRF2 10 CRF2 S1 CRF2-10 CRF2-S1 CRF2X Cytokine receptor class II member 10 Cytokine receptor class-II member 10 Cytokine receptor family 2 member 10 Cytokine receptor family type 2 Cytokine receptor family type 2 soluble 1 I22R2_HUMAN IL 22 receptor subunit alpha 2 IL 22R alpha 2 IL-22 receptor subunit alpha-2 IL-22BP IL-22R-alpha-2 IL-22RA2 IL22 RA2 IL22BP IL22R alpha 2 Il22ra2 Interleukin 22 binding protein Interleukin 22 Receptor alpha 2 Interleukin-22 receptor subunit alpha-2 Interleukin-22-binding protein MGC150509 MGC150510 soluble 1 ZcytoR16 |
Images
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Fig1:
Western blot analysis of IL22 RA2 on different lysates with Rabbit anti-IL22 RA2 antibody (HA500365) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Human lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500365) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IL22 RA2 antibody (HA500365) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500365) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling IL22 RA2 with Rabbit anti-IL22 RA2 antibody (HA500365) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL22 RA2 antibody (HA500365) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.