alpha Tubulin Rabbit Polyclonal Antibody
cat.: HA500366
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.9ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TBA3C aa 151-350 / 865. |
| Positive control: | HeLa cell lysate, NIH/3T3 cell lysate, A431, SH-SY5Y, human testis tissue, mouse testis tissue. |
| Subcellular location: | Cytoskeleton |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P68366 Human | P68368 Mouse | Q5XIF6 Rat |
| Alternative names: | Alpha-tubulin 1 ALS22 B ALPHA 1 bA408E5.3 H2 ALPHA Hum a tub1 Hum a tub2 LIS3 MGC171407 MGC55332 TBA4A_HUMAN Testis-specific alpha-tubulin TUBA1 TUBA1A tuba1l Tuba4a Tubulin alpha 1 chain Tubulin alpha Tubulin alpha-1 chain tubulin alpha-1B chain Tubulin alpha-4A chain Tubulin H2-alpha Tubulin, alpha 1 (testis specific) tubulin, alpha 1, like Tubulin, alpha 4a Tubulin, alpha, testis-specific Tubulin, alpha-1 |
Images
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Fig1:
Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 5 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500366, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 50 kDa |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of SH-SY5Y cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.