NINJ1/Ninjurin-1 Rabbit Polyclonal Antibody
cat.: HA500367
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.3ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Ninjurin-1 aa 1 – 80 (Extracellular). |
| Positive control: | Human liver tissue lysate, RAW264.7 cell lysate, mouse kidney tissue lysate, mouse liver tissue lysate, human lung tissue lysate, A549 cell lysate, LNCAP cell lysate. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q92982 Human |
| Alternative names: | Nerve injury induced protein 1 Nerve injury-induced protein 1 NIN1 NINJ1 NINJ1_HUMAN NINJURIN Ninjurin-1 |
Images
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Fig1:
Western blot analysis of NINJ1/Ninjurin-1 on different lysates with Rabbit anti-NINJ1/Ninjurin-1 antibody (HA500367) at 1/1,000 dilution. Lane 1: Human liver tissue lysate (40 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NINJ1/Ninjurin-1 on different lysates with Rabbit anti-NINJ1/Ninjurin-1 antibody (HA500367) at 1/500 dilution. Lane 1: Human liver tissue lysate (20 µg/Lane) Lane 2: Human lung tissue lysate (20 µg/Lane) Lane 3: A549 cell lysate (10 µg/Lane) Lane 4: LNCAP cell lysate (10 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500367) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.