YWHAZ Rabbit Polyclonal Antibody
cat.: HA500368
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.5ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human YWHAZ aa 50-245. |
| Positive control: | HepG2 cell lysate, RAW264.7 cell lysate, human kidney tissue lysate, SiHa cell lysate, A549 cell lysate, A375 cell lysate, Hela cell lysate, SH-SY5Y cell lysate, Raji cell lysate, PC-12 cell lysate, mouse lung tissue lysate, rat large intestine tissue, mouse epididymis tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:600 |
| Uniprot #: | SwissProt: P63104 Human | P63101 Mouse Entrez Gene: 25578 Rat |
| Alternative names: | 14-3-3 protein zeta/delta Protein kinase C inhibitor protein 1 KCIP-1 YWHAZ |
Images
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Fig1:
Western blot analysis of YWHAZ on different lysates with Rabbit anti-YWHAZ antibody (HA500368) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: RAW264.7 cell lysate (10 µg/Lane) Lane 3: Human kidney tissue lysate (20 µg/Lane) Lane 4: SiHa cell lysate (10 µg/Lane) Lane 5: A549 cell lysate (10 µg/Lane) Lane 6: A375 cell lysate (10 µg/Lane) Lane 7: Hela cell lysate (10 µg/Lane) Lane 8: SH-SY5Y cell lysate (10 µg/Lane) Lane 9: Raji cell lysate (10 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500368) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of YWHAZ on different lysates with Rabbit anti-YWHAZ antibody (HA500368) at 1/5,000 dilution. Lane 1: PC-12 cell lysate (10 µg/Lane) Lane 2: Mouse lung tissue lysate (20 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500368) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-YWHAZ antibody (HA500368) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500368) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-YWHAZ antibody (HA500368) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500368) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-YWHAZ antibody (HA500368) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500368) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.