YWHAZ Rabbit Polyclonal Antibody
cat.: HA500368
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.5ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human YWHAZ aa 50-245. |
| Positive control: | Hels cell lysate, A549 cell lysate, Hep G2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat large intestine tissue, mouse epididymis tissue, mouse testis tissue, PC-12. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000-1:5,000 1:600 1:100 1:1,000 |
| Uniprot #: | SwissProt: P63104 Human | P63101 Mouse Entrez Gene: 25578 Rat |
| Alternative names: | 14-3-3 protein zeta/delta Protein kinase C inhibitor protein 1 KCIP-1 YWHAZ |
Images
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Fig1:
Western blot analysis of YWHAZ on different lysates with Rabbit anti-YWHAZ antibody (HA500368) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 4: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500368, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 28 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-YWHAZ antibody (HA500368) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500368) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-YWHAZ antibody (HA500368) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500368) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-YWHAZ antibody (HA500368) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500368) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of PC-12 cells labeling YWHAZ with Rabbit anti-YWHAZ antibody (HA500368) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YWHAZ antibody (HA500368) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Flow cytometric analysis of PC-12 cells labeling YWHAZ. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500368, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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