RANKL Rabbit Polyclonal Antibody
cat.: HA500369
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RANKL aa 100-317. |
| Positive control: | Hela cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela. |
| Subcellular location: | Cell membrane, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: O14788 Human | O35235 Mouse | Q9ESE2 Rat |
| Alternative names: | CD254 hRANKL2 ODF OPGL OPTB2 Osteoclast differentiation factor Osteoprotegerin ligand Rank Ligand RANKL Receptor activator of nuclear factor kappa B ligand Receptor activator of nuclear factor kappa-B ligand sOdf TNF related activation induced cytokine TNF-related activation-induced cytokine TNF11_HUMAN TNFSF 11 Tnfsf11 TRANCE Tumor necrosis factor (ligand) superfamily member 11 Tumor necrosis factor ligand superfamily member 11 Tumor necrosis factor ligand superfamily member 11, soluble form |
Images
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Fig1:
Western blot analysis of RANKL on different lysates with Rabbit anti-RANKL antibody (HA500369) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Mouse spleen tissue lysate (20 µg/Lane) Lane 3: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500369) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling RANKL with Rabbit anti-RANKL antibody (HA500369) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RANKL antibody (HA500369) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.