PPP3CB Rabbit Polyclonal Antibody
cat.: HA500370
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.8ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PPP3CB aa 475-524. |
| Positive control: | Human brain tissue lysate, rat brain tissue lysate, rat brain tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:600 |
| Uniprot #: | SwissProt: P16298 Human | P48453 Mouse | P20651 Rat |
| Alternative names: | Calcineurin A beta Calcineurin A2 Calcineurin B, formerly Calmodulin dependent calcineurin A subunit beta isoform Calmodulin-dependent calcineurin A subunit beta isoform CALNA 2 CALNA2 CALNB CAM PRP catalytic subunit CAM-PRP catalytic subunit CNA2 CnAbeta PP2BB_HUMAN PP2Bbeta PPP3CB Protein phosphatase 2B, catalytic subunit, beta isoform, formerly Protein phosphatase 3 (formerly 2B) catalytic subunit beta isoform Protein phosphatase 3 catalytic subunit beta isoform Protein phosphatase 3 catalytic subunit beta isozyme Serine/threonine protein phosphatase 2B catalytic subunit beta isoform Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform |
Images
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Fig1:
Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (HA500370) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500370) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PPP3CB antibody (HA500370) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500370) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-PPP3CB antibody (HA500370) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500370) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.