RPS27A Rabbit Polyclonal Antibody
cat.: HA500371
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.98ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RPS27A aa 77-150. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, HepG2 cell lysate, rat liver tissue lysate, human kidney tissue, A172, A375. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:600 1:200 |
| Uniprot #: | SwissProt: P62979 Human | P62983 Mouse | P62982 Rat |
| Alternative names: | 40S ribosomal protein S27a CEP 80 CEP80 HUBCEP 80 HUBCEP80 Ribosomal protein S27a RPS 27A UBA 80 UBA80 UBCEP 1 UBCEP 80 UBCEP1 UBCEP80 Ubiquitin 40S ribosomal protein S27a Ubiquitin and ribosomal protein S27a Ubiquitin carboxyl extension protein 80 Ubiquitin CEP80 |
Images
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Fig1:
Western blot analysis of RPS27A on different lysates with Rabbit anti-RPS27A antibody (HA500371) at 1/500 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: HepG2 cell lysate (10 µg/Lane) Lane 4: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 18 kDa Observed band size: 15 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500371) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RPS27A antibody (HA500371) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500371) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of A172 cells labeling RPS27A with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of A375 cells labeling RPS27A with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RPS27A antibody (HA500371) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.