MLST8 Rabbit Polyclonal Antibody
cat.: HA500372
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MLST8 aa 1-250. |
| Positive control: | C2C12 cell lysate, L6 cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, 293 cell lysate, MCF-7 cell lysate, PC-3M, rat heart tissue, mouse skeletal muscle tissue, PC-3. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:800 1:1,000 |
| Uniprot #: | SwissProt: Q9BVC4 Human | Q9DCJ1 Mouse | Q9Z2K5 Rat |
| Alternative names: | G protein beta subunit like G protein beta subunit-like Gable GbetaL GBL GBL protein LST8 LST8_HUMAN Mammalian lethal with SEC13 protein 8 MGC111011 mLST8 MTOR associated protein LST8 homolog (S. cerevisiae) POP3 POP3 homolog (S. pombe) Protein GbetaL Target of rapamycin complex subunit LST8 TORC subunit LST8 WAT1 WAT1 homolog (S. pombe) |
Images
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Fig1:
Immunocytochemistry analysis of PC-3M cells labeling MLST8 with Rabbit anti-MLST8 antibody (HA500372) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MLST8 antibody (HA500372) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-MLST8 antibody (HA500372) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500372) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-MLST8 antibody (HA500372) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500372) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of PC-3 cells labeling MLST8. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500372, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Western blot analysis of MLST8 on different lysates with Rabbit anti-MLST8 antibody (HA500372) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500372, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.