RRAGA Rabbit Polyclonal Antibody
cat.: HA500373
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.85ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RRAGA aa 1-250. |
| Positive control: | 293 cell lysates, RAW264.7 cell lysate, mouse lung tissue lysate, rat lung tissue lysate. |
| Subcellular location: | Lysosome, Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1-500-1:2,000 |
| Uniprot #: | SwissProt: Q7L523 Human | Q80X95 Mouse Entrez Gene: 117044 Rat |
| Alternative names: | Adenovirus E3 14.7 kDa-interacting protein 1 FIP-1 Rag A RagA Ras-related GTP-binding protein A RRAGA RRAGA_HUMAN |
Images
|
Fig1:
Western blot analysis of RRAGA on 293 cell lysates with Rabbit anti-RRAGA antibody (HA500373) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 36.5 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500373) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of RRAGA on different lysates with Rabbit anti-RRAGA antibody (HA500373) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate (10 µg/Lane) Lane 2: Mouse lung tissue lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 36.5 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500373) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.