HA500374

DDX58 Rabbit Polyclonal Antibody

cat.: HA500374

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P, IF-Cell, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2.1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 107 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human DDX58 aa1-250.
Positive control:	A431 cell lysate, SiHa cell lysate, rat bladder tissue, human kidney tissue, human stomach tissue, mouse large intestine tissue, Hela, A549.
Subcellular location:	Cytoskeleton, Plasma membrane, Cytoplasm.
Recommended Dilutions: WB IHC-P IF-Cell FC	1:1,000 1:400-1:1,000 1:200 1:500-1:1,000
Uniprot #:	SwissProt: O95786 Human \| Q6Q899 Mouse
Alternative names:	Ddx58 DDX58_HUMAN DEAD (Asp Glu Ala Asp) box polypeptide 58 DEAD (Asp Glu Ala Asp/His) box polypeptide DEAD box protein 58 DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 DKFZp434J1111 DKFZp686N19181 FLJ13599 Probable ATP dependent RNA helicase DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid inducible gene 1 protein Retinoic acid-inducible gene 1 protein Retinoic acid-inducible gene I protein RIG I Rig-1 RIG-I RIG1 rigi RLR 1 RNA helicase RNA helicase RIG I SGMRT2

Images

	Fig1: Western blot analysis of DDX58 on different lysates with Rabbit anti-DDX58 antibody (HA500374) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500374) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-DDX58 antibody (HA500374) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500374) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-DDX58 antibody (HA500374) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500374) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-DDX58 antibody (HA500374) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500374) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-DDX58 antibody (HA500374) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500374) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunocytochemistry analysis of Hela cells labeling DDX58 with Rabbit anti-DDX58 antibody (HA500374) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-DDX58 antibody (HA500374) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Fig7: Flow cytometric analysis of A549 cells labeling DDX58.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500374, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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