PSME4 Rabbit Polyclonal Antibody
cat.: HA500375
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.57ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 211 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSME4 aa 1,550-1,843. |
| Positive control: | 293T cell lysate, Hela cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, rat skeletal muscle tissue lysate, rat liver tissue, mouse liver tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: Q14997 Human | Q5SSW2 Mouse Entrez Gene: 498433 Rat |
| Alternative names: | KIAA0077 PA200 Proteasome Activator 200 kDa Proteasome activator complex subunit 4 Proteasome activator PA200 PSME 4 psme4 PSME4_HUMAN |
Images
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Fig1:
Western blot analysis of PSME4 on different lysates with Rabbit anti-PSME4 antibody (HA500375) at 1/500 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: RAW264.7 cell lysate (10 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (20 µg/Lane) Predicted band size: 211 kDa Observed band size: 211 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500375) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PSME4 antibody (HA500375) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500375) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PSME4 antibody (HA500375) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500375) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.