HA500376

ELKS Rabbit Polyclonal Antibody

cat.: HA500376

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2.39ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 128 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human ELKS aa 850-1,116.
Positive control:	HEK-293 cell lysate, A549 cell lysate, SiHa cell lysate, NIH/3T3 cell lysate, rat testis tissue, human liver tissue, human kidney tissue, human placenta tissue, mouse large intestine tissue, A375, SiHa.
Subcellular location:	Cytoplasm, cytoskeleton, microtubule organizing center, centrosomem, Cytoplasm, Membrane, Golgi apparatus membrane, Cell junction, synapse, presynaptic cell membrane.
Recommended Dilutions: WB IHC-P IF-Cell	1:5000 1:800 1:200
Uniprot #:	SwissProt: Q8IUD2 Human \| Q99MI1 Mouse \| Q811U3 Rat
Alternative names:	Cast 2 CAST2 CAZ associated structural protein 2 Elks ELKS/RAB6 interacting/CAST family member 1 ELKS/RAB6-interacting/CAST family member 1 ERC 1 ERC protein 1 ERC-1 Erc1 ERC1 protein KIAA1081 MGC12974 RAB6 interacting protein 2 RAB6-interacting protein 2 RAB6IP2 RB6I2_HUMAN

Images

Fig1: Western blot analysis of ELKS on different lysates with Rabbit anti-ELKS antibody (HA500376) at 1/5,000 dilution.

Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 3: SiHa (Human cervical cancersSquamous cell) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 59 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500376, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 128 kDa
Observed band size: 128 kDa

Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunocytochemistry analysis of A375 cells labeling ELKS with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
	Fig8: Immunocytochemistry analysis of SiHa cells labeling ELKS with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.