DAB2P Rabbit Polyclonal Antibody
cat.: HA500378
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2.0ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 132 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DAB2IP aa 950-1189. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, Hela cell lysate, A431 cell lysate, human thyroid carcinoma tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, Cytoplasm. |
| Recommended Dilutions:
IHC-P IF-Cell |
1:800 1:200 |
| Uniprot #: | SwissProt: Q5VWQ8 Human | Q3UHC7 Mouse | Q6P730 Rat |
| Alternative names: | AF9Q34 AIP1 ASK interacting protein ASK interacting protein 1 ASK1 interacting protein 1 DAB2 interacting protein DAB2 interaction protein DAB2P DIP1/2 Disabled homolog 2 interacting protein DOC 2/DAB2 interactive protein FLJ39072 KIAA1743 nGAP like protein |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-DAB2P antibody (HA500378) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500378) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling DAB2P with Rabbit anti-DAB2P antibody (HA500378) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-DAB2P antibody (HA500378) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.